Answer:
Given:
Number of colonies on plate = 40
Amount of DNA used for transformation = 100ng
Volume of competent cells used = 100ul
Total volume = 1ml (1000ul)
Volume plated for growth = 200ul
The amount of cells transformed per 1 µg of DNA is called the transformation efficiency
(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA
Amount of DNA used for plating = 100ng * (200/1000)
= 100ng * (1/5)
= 100/5 = 20ng
Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng
(2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA
Transformation efficiency = 2000 Transformants / ug DNA
= 2 x 10³ Transformants / ug DNA
Explanation:
Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.
<span>Alleles are each of two or more alternative forms of a gene that are found at the same position in a chromosome. Traits on the other hand, are the characteristics that are defined by genes. So the combination of alleles for a gene is what manifests itself into a trait.</span>
Cell membrane is like doors and windows!!
if you know how; comment !
Answer:
Pollination
Explanation: If you have ever seen bee movie, you know that pollen is the most essential ingredient to flowers, which is transported and spread by bee's. if there were no more bees, there would be no way to pollinate the flowers, causing them to wither and die. Fortunately, there are other pollinators, but bees are one of the most important.
