Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
The pad of fatty tissue that covers the area of the pubic bone is called the mons pubis
The pubic bone is protected by a fatty cushion called the mons pubis. The mons, or mons veneris in females, is another name for it. Although both have a mons pubis, females tend to have it more pronounced.
Females tend to have a large mound of tissue that is typically covered in pubic hair. During sexual contact, the mons pubis serves as a source of cushioning. Sebaceous glands that release pheromones to promote physical attraction are also found in the mons pubis.
The mons pubis is a part of the vulva. This is the rounded region in the bottom portion of the belly that is in front of the pubic bones (abdomen). At puberty, it grows hair all over it. The vulva has two skin folds.
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I think the correct answer would be diarrhea. It is often a result of consumption of contaminated food and drinks. And each year, it was estimated that approximately 2 million children died due to this. It is a condition where bowel movements are frequent and is in liquid form which would lead to dehydration.
Answer:
<u><em>Phosphorus</em></u>
Phosphorus is a chemical element with the symbol P and atomic number 15. Elemental phosphorus exists in two major forms, white phosphorus and red phosphorus, but because it is highly reactive, phosphorus is never found as a free element on Earth.
Explanation:
Brainliest?
Answer:
the eagle habitat is estuaries, large lakes, reservoirs, rivers, and some seacoasts, but in the winter the eagle congregate near open water in tall trees for spotting prey.
Explanation:
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