The biosphere is made up of all living things (organisms).
The biosphere includes animals, biomes, population, communities, and ecosystems. The biomes are climate regions which can contain certain types of animals and plants. An ecosystem is all living things in one specific area, also such as plants, animals, and organisms.
A gecko, fly agaric (fungus), and a palm tree all have cells with nuclei, but a gecko and fly agaric are both heterotrophic (making them more closely related) while a palm tree is a photosynthetic autotroph.
b. the first stage because it could never be in the secound stage