Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
2. To focus a microscope using the high power lens you must first in low power center the specimen and make sure it is in focus. Then switch to high power and adjust using the fine adjustment or the smaller knob.
3. It prevents dust from settling on the scope and in the lenses.
A single molecule is the one that is responsible for initiating a signal transduction pathway. The answer in this question is a single molecule. Molecule is defined as an electrically neutral group of two atoms or more held together by a chemical bonds.
Answer:A
Explanation:it makes sense
