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charle [14.2K]
3 years ago
12

The DNA found in the nucleus is responsible for directing the

Biology
1 answer:
Lunna [17]3 years ago
3 0
The Correct answer is B
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In coral reefs, clownfish live unharmed among the poisonous tentacles of sea anemones. The sea anemones protect the clownfish fr
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The sea anemone and the clownfish live together in a type of symbiotic relationship called mutualism, where both species benefit from the other. The sea anemone offers the clownfish protection and leftover food

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Who performed experiments using enzymes to identify DNA as genetic material?
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When plants use sunlight to make food, the energy of sunlight is a what?
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3 years ago
The kinetics analyses of an enzyme are measured as a function of substrate concentration in the presence and in the absence of 2
Aleksandr-060686 [28]

Answer:

take the reciprocal of the values as 1/S and 1/V and plot the values on lineweaver-burk plot.

so the re-arranged data is as following

1/S               1/V (no inhibitor)       1/V (with inhibitor)

0.333           0.096                       0.244

0.2              0.068                         0.156

0.1               0.044                         0.088

0.033          0.029                        0.044

0.011            0.024                         0.029

and the plotted lineweaver-burk plot with the equation of straight line would be....

where, first line represents the velocity of reaction with no inhibitor and second line represent the velocity in presence of inhibitor. X-axis has the substrate concentration as 1/S and y-axis has the velocity as 1/V.

as per the plot, equation of line on the lineweaver-burk plot, Y = mx + c, where c = 1/Vmax and m = Km/Vmax.

so solve for the given equations.

First, equation of line in the absence of inhibitor is Y = 0.23x + 0.021

so Vmax = 1/0.021 = 47.6 micromol/min

and Km/Vmax = 0.23, or Km = 0.23 x 47.6

Km = 10.95

Km do not have a unit, so 10.95 x 10-6 or as per your values round it up to 1 decimal point as 1.1 x 10-5

so value of Vmax = 47.6micromol/min and Km = 1.1 x 10-5 in the absence of inhibitor.

similarly, equation of line in the presence of inhibitor is given as Y = 0.67x + 0.021

so Vmax = 1/0.021 = 47.6 micromol/min

and Km/Vmax = 0.67

or Km = 0.67 x Vmax = 0.67 x 47.6 = 31.89

Km do not have a unit, so 31.89 x 10-6 or 3.1 x 10-5

so value of Vmax = 47.6micromol/min and Km = 3.1 x 10-5 in the absence of inhibitor.

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