In an investigation of the cycling of environmental gases, a student placed water and bromthymol blue in each of four test tubes as shown in the diagrams below. No additional items were placed in tube 1, a snail was placed in tube 2, an aquatic plant (elodea) was placed in tube 3, and both a snail and an elodea were placed in tube 4. The tubes were then stoppered and placed in bright light for 24 hours. The solution in tube 3 changes after 24 hours because it would contain less nitrogen.
The dfferent tubes are shown in the picture that I uploaded.