Answer:
write the procedure in the passive voice (reporting fashion )
Answer:
(A) It prevents electron flow from the iron-sulfur centers in complex 1 to the ubiquinone. Due to reduction in electron transfer rate, there is a decrease in the production of ATP which is dangerous for some insects and fish over time.
(B) It also prevents electron flow from cytochrome b to cytochrome c1 at the complex III which leads to QH2 accumulation. If oxidized Q is not present, these is alteration of electron flow and the production of ATP is altered.
(C) Rotenone only prevent electron transfer into the chain at Complex 1 but it does not affect electron transfer at Complex II. Although there is slow ETC, it does not stop completely. However, Antimycin A prevents the oxidation of QH2, the final electron acceptor crom complex I and complex II. Thereby, stopping the production of both ETC and ATP. It can be concluded that antimycin A is a more potent poison.
Explanation:
Rotenone prevents electron flow from the iron-sulfur centers in complex 1 to the ubiquinone. Due to a reduction in electron transfer rate, there is a decrease in the production of ATP which is dangerous for some insects and fish over time. Antimycin A also prevents electron flow from cytochrome b to cytochrome c1 at the complex III which leads to QH2 accumulation. If oxidized Q is not present, there is an alteration of electron flow and the production of ATP is altered. Antimycin A is more potent than rotenone.
In the mitochondria, the powerhouse of the cell.
WORKING DISTANCE is space between the bottom of objective the lens and the surface of the slide.
The distance between front edge of the objective and the lens and the specimen surface ("with the surface of the cover glass in case of the cover glass objective lens") when the specimen is also focused.
The working distance (W.D.) which is determined by the linear measurement of the objective and front lens to the focal plane. In the general, the objective of the working distance decreases by the magnification and the numerical aperture of both increase. The working distance is the distance and from the front of the lens to be the focal plane
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