Answer:
C.The added epitope disrupts the function of the tagged protein
Explanation:
When an additional sequence is tagged to a protein to use comercial antibodies, there are several reasons why this procedure wouldn't work as expected (note that we're assuming the protein is being expressed but it's not possible to detect it).
For example, the sequence of nucleotide added to codify for the tagged epitope are removed during the RNA processing. In that case, the protein would be expressed without the epitope, so it would be impossible to localize it with the antibodies.
Also, it could be that the new epitope is affecting some way the protein folding, making it not functional. This way, it would be degraded by the cell so it wouldn't be detected.
Another possibility is that the epitope doesn't affects the protein folding nor its function, but during the folding ends up in a conformation that makes it inaccesible for the antibody.
In summary, the way as the possible answers to this question are shown, the correct option seems to be C:<em> The added epitope disrupts the function of the target protein.</em>
The Richter scale is a way of giving the intensity of all earthquakes a numerical value. this allows the strengths of different earthquakes to be compared. Because of this, i think the best answer is D
Answer:
Control group: volunteer stationary bike riders in a pollution-free chamber
Experimental group: volunteer stationary bike riders in a chamber filled with air pollutants common to Los Angeles
Independent variable: exposure to air pollution
Dependent variable: The heart rates (physical fitness) of the volunteer riders.
Explanation:
In a scientific experiment, data is compared between the control group and experimental group to ascertain the effect of the independent variable on the dependent variable. A control group is the group in an experiment that is not treated with the independent variable i.e. the independent variable is controlled while the experimental group is the group that is treated with an independent variable.
N.B: The independent variable is the variable controlled by the experimenter in order to influence the dependent variable (measurable outcome).
In this experiment, the independent variable is the AIR POLLUTION the volunteer riders are exposed to while the dependent variable is the PHYSICAL FITNESS/ HEART RATES of the volunteer riders, which is dependent on the exposure to air pollution (independent variable).
The control group in this experiment is the volunteer riders placed in a POLLUTION-FREE CHAMBER where the independent variable cannot influence or have any effect on the measurable variable (dependent). The experimental group is the volunteer riders placed in a CHAMBER FILLED WITH AIR POLLUTANTS. The independent variable (air pollution) has been changed in the experimental group.
Hypotonic - because there is a higher concentration inside the cell than outside of it
