Redundant mechanisms
To maintain homeostasis, redundant mechanisms are present in the body so that when one of the systems fails, other complementary or backup systems can help to compensate and ensure the survive of the organism by maintaining homeostasis. Examples of these redundant systems involve blood pressure regulation, temperature regulation, breathing and pH control in the body during exercise.
Avogadro's number tells us there are 6.022 x 1023 molecules of water per mole of water. So, next we calculate how many molecules there are in a drop of water, which we determined contains 0.002775 moles: molecules in a drop of water = (6.022 x 1023 molecules/mole) x
Proximal convoluted tubule cells. As well as the epithelial cells along the renal tubule and the duct. Hope this helps!:)
Answer:
Use a step wise process
Explanation:
In order to find the presence of the recombinant protein:
- Culture the cells that you believe harbors the plasmid for the recombination protein (+) and also cells that do not (-). This is your control.
- Extract the plasmid from both strains.
- Observe the plasmid map and the area of your insert. Use this to select the restriction enzymes at the beginning and the end of the YFG gene. You can also use a restriction site inside the gene but not in the plasmid for better control.
- Perform the restriction enzyme digest on both (+) and (-) according to your protocol and separate using gel electrophoresis.
- Observe the separate bands that you see. In the (+) there should be a band that is the size of your insert and a larger band that is the size of your plasmid control band and in the (-) there should be a single band that is the size of the plasmid but non the size of the insert.
