1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
mel-nik [20]
3 years ago
8

What is the best explanation if the gel of your PCR product shows smeared bands of multiple sizes? Select one:

Biology
1 answer:
trapecia [35]3 years ago
8 0

Answer:

e. None of the above

Explanation:

For me as a Researcher, the reason could be increased Concentration of your DNA sample which you are using as your template. Try to decrease the concentration of DNA (up to 100 ng per reaction is enough and can increase up to 200 ng). so the reason for getting non specific bands is increase concentration of DNA  which results in non specific amplification and also degradation of DNA in the reaction which you can see in your gel electrophoresis results.  

i always corrected my results using the same technique that is lowering the concentration of DNA between 100 and 200 ng per single reaction of PCR.

You might be interested in
• bahrami, m. et al. deep feature extraction of single-cell transcriptomes by generative adversarial network. bioinformatics (ox
HACTEHA [7]

Single-cell RNA-sequencing (scRNA-seq) provides the chance to analyze heterogeneous cellular compositions and probe the patterns of gene expression that are unique to each cell type under various circumstances. However, batch effects like lab setups and individual variability make it difficult to use them in cross-condition designs.

<h3>What is Single-cell transcriptomes ?</h3>

In single-cell transcriptomics, the messenger RNA levels of hundreds to thousands of genes are simultaneously measured to assess the degree of gene expression in individual cells within a particular population.

<h3>Advantages : </h3>

• Integrated protocol proceeds directly from whole cells and preserves sample integrity.

• High resolution analysis enables discovery of cellular differences typically hidden by bulk sampling methods.

• Robust transcriptome analysis down to single-cell input levels for high-quality samples.

To know more about Single-cell transcriptome please click here : brainly.com/question/28187739

#SPJ4

4 0
2 years ago
As the atmosphere and Earth’s surface materials absorb ultraviolet light, visible light, and infrared energy, they gain thermal
aliina [53]

Answer: The answer is B

Explanation:

5 0
3 years ago
Scientist have evidence that media right have come from which location
dimaraw [331]

Answer:

Please mark as brainlist.

Explanation:

Scientific evidence for warming of the climate system is unequivocal.

6 0
3 years ago
5. What is the difference between mitochondria and chloroplast?
FinnZ [79.3K]

Answer:

C. Mitochondria convert sugars into energy, while chloroplasts carry out

photosynthesis.

Explanation:

Chloroplasts have the ability to use light to convert carbon, derived from carbon dioxide into sugar. Mitochondria on their part break down simple sugars into carbon dioxide and release energy. Chloroplasts are larger and have greater complexity than mitochondria, and they have several critical functions to perform, besides the generation of ATP. Apart from converting carbon dioxide to carbohydrates they synthesize amino acids, fatty acids, and the lipid contained in their own membranes.

Mitochondria are to be found in both plant and animal cells, whereas chloroplasts are found only in plant cells. The former have a structure composed of a prokaryotic cell, whereas Chlorplasts are made up of stacks of thylakoids surrounded by a fluid called stroma. According to some theories, mitochondria exist because of endocytosis of aerobic bacteria, while chloroplasts are around because of the result of endocytosis of photosynthetic bacteria.

Chloroplasts exist only in plant cells and lend the green color of most plants. Mitochondria on the other hand are found in both animal and plant cells and are engaged in the production of ATP. To use simple layman language, mitochondria in animal cells changes energy into forms that animals can use, while chloroplasts in plant cells convert sunlight into energy that plants can use. From this it follows that mitochondria in a sense are the power plant in the animal cells, because it generates energy. Chloroplasts provide the green color to the plant due to the chlorophyll present in them.

Mitochondria cells are 1 to 10 um long in length. These can change shapes, get around, and divide into two. The cell is surrounded by an envelope of two membranes. The membrane on the outside is smooth while the other one is marked by what are called cristae.

Photosynthesis happens only in plants. This is because only plants contain chloroplasts. Thus we can see that mitochondria and chloroplasts are the building blocks of life for plants and animals respectively in the sense that they provide nourishment to the organisms. The two are also representative of the dividing line between the two kingdoms of life on earth-the animal and the plant.

These two structures are markers of two philosophies of life form if one may use the use the term. One that is self sustaining, manufacturing its own food, the other that is dependent upon the former as the primary source of food, but far more complex and evolved way of life in many other aspects.

4 0
3 years ago
90 POINT HELP ASAP!!!!!!!!!!!
-BARSIC- [3]

Answer:

Lungs

Explanation:

3 0
3 years ago
Read 2 more answers
Other questions:
  • Describe the connection between fossil fuel burning, the melting of polar ice, and the rising of global sea levels.
    13·1 answer
  • Can organisms create their own energy? Explain your answer
    6·1 answer
  • Which structure in the female reproductive system is comparable to the external reproductive organs in males?
    9·2 answers
  • All living organisms can be categorized by their response to atmospheric oxygen as obligate aerobes, facultative aerobes, obliga
    12·1 answer
  • What is a major difference between mitosis and meiosis I in a diploid organism?
    13·2 answers
  • What is the thing in the back of your throat called
    6·1 answer
  • A term used to describe organisms that cause disease​
    9·2 answers
  • Help me immediately please​
    12·1 answer
  • The picture below shows the bone structures of whale and bird.
    8·1 answer
  • “The movement of cytoplasm is due to the movement of chloroplasts in the living cells of onion plant”, “while the movement of ch
    6·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!