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mel-nik [20]
3 years ago
8

What is the best explanation if the gel of your PCR product shows smeared bands of multiple sizes? Select one:

Biology
1 answer:
trapecia [35]3 years ago
8 0

Answer:

e. None of the above

Explanation:

For me as a Researcher, the reason could be increased Concentration of your DNA sample which you are using as your template. Try to decrease the concentration of DNA (up to 100 ng per reaction is enough and can increase up to 200 ng). so the reason for getting non specific bands is increase concentration of DNA  which results in non specific amplification and also degradation of DNA in the reaction which you can see in your gel electrophoresis results.  

i always corrected my results using the same technique that is lowering the concentration of DNA between 100 and 200 ng per single reaction of PCR.

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Explanation:

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Answer:

A glance of earth taken from space will depict it blue. This blue colour is actually water, the major part of the earth is covered with water. We need water for almost everything, for example- drinking, bathing, cooking etc and therefore we should know about the properties of water. 65 % human body is composed of water. Water is essential for the survival of life on earth. Water is distributed unevenly on the earth’s surface. It forms a major solvent and dissolves almost every polar solute. So let us have a look at its properties.

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Example:

Acidic Behaviour: H2O(l)+NH3(aq) ⇌ H3O+(aq)+NH+4(aq)

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Water has a very strong hydrating tendency due to its dielectric constant. It dissolves many ionic compounds. Some covalent and ionic compounds can be hydrolyzed in water.

Explanation:

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