Answer:
The correct answer is: d. REs activity is best preserved by freezing the RE and thawing it out for brief periods of time as needed.
Explanation:
- Restriction enzymes can be defined as endonucleases that can recognize specific nucleotide sequence in a DNA (Deoxyribonucleic acid) sequence and cause breakage of phosphodiester bond in the DNA at the target site. Hence, it has the capacity of cleaving a DNA sequence.
- These are made up of proteins which are required to be present in the accurately folded conformation in order to function.
- Any form of alteration in the three-dimensional structure of the protein induced due to physical, chemical, mechanical or physiological factors can cause denaturation of the protein which in turn can render the protein non-functional.
- Freezing of restriction enzymes (REs) directly without the use of glycerol will cause the movement of water molecules within the proteins as well layering of water molecules on the protein surface.
- At low temperature these water molecules change to ice crystals which can alter the conformation of the protein or can cause breakage of peptide bonds in the proteins by physical force.
- This can cause the protein to get denatured and hence non-functional.
- Hence, it is never advisable to preserve REs by freezing directly without glycerol.
- Glycerol forms a layer around the protein molecules and prevents the protein molecules from coming into direct contact with the water molecules and hence prevents protein denaturation.
Answer:
The body uses sugars from carbohydrates which supply the brain with glucose as the brain uses it as a "fuel source".
<h2>Why is glucose so important for the brain?</h2>
Quick answer: It takes a lot of energy to receive, interpret, and send signals via your neurons. Glucose is the simples sugar that can be used to make energy.
Cells require energy to carry out their typical everyday tasks. The simplest sugar that our cells can utilize for energy is glucose. Since your neurons are specialized cells, many additional cells are also present to support or protect them. All of the senses you can experience utilizing incoming neurons (from the body to the brain) are transmitted to and interpreted by the brain, including touch, pain, vibration, temperature, smell, sight, hearing, taste, and others. Signal reception and interpretation need energy. Additionally, your brain instructs your body to "do" things, which uses energy. Additionally, you spend a significant portion of your waking hours "thinking," which consumes energy. This explains why 20% of the glucose in your body is used by our teeny, tiny, little brains.
Thank you,
Eddie
Enzymes have an attachment site called the active site for the enzyme and substrate <span>to join.
Happy studying ^-^</span>
Thanks ya...✌✌
<em>the process by which green plants and some other organisms use sunlight to synthesize nutrients from carbon dioxide and water. Photosynthesis in plants generally involves the green pigment chlorophyll and generates oxygen as a by-product.</em>
