When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible restriction enzymes. An enzyme, DNA ligase, then covalently binds the plasmid to the new fragment thereby generating a complete, circular plasmid that can be easily maintained in a variety of biological systems. Read on for an in-depth breakdown of how to do perform restriction digests.
In spherocytosis, there is a defect in the membrane proteins of the red blood cells, specifically ankyrin and spectrin. These membrane proteins contribute to the biconcave shape of red blood cells therefore the loss of these proteins will lead the red blood cells to lose its biconcave shape--leading to abnormally shaped red blood cells (spheres) hence the name. This can lead to premature destruction of red blood cells and jaundice due to hyperbilirubinemia. Spherocytes do not hold oxygen and carbon dioxide well as spherocytes have a decreased surface area.
Answer:
Lungs.
Explanation:
Lungs are the organs that are responsible for gaseous exchange in humans. The lungs absorb oxygen from the air that is required for the process of respiration while on the other hand, carbon dioxide is transferred from the cells to the lungs through blood because carbondioxide gas is a waste material that is produced during respiration so it can be removed from the body with the help of lungs..
Answer:
Magma is less dense than the surrounding rock which causes it to rise. When magma reaches the surface it is then called lava and the eruptions of lava and ash produce volcanoes. The lava that reaches the Earth's surface will harden and become igneous rock.
Explanation:
