Question

To calculate the turnover number of an enzyme, you need to know: A) the enzyme concentration. B) the initial velocity of the catalyzed reaction at [S] >> Km. C) the initial velocity of the catalyzed reaction at low [S]. D) the Km for the substrate. E) both A and B.

Answer 1

Answer is option "E"

Explanation:

• Enzymes belong to the proteins category because enzymes are long chains of amino acids bound together by peptide bonds
• Enzymes have a special marker on them called an active site that, like a puzzle piece, fits with only one other type of molecule, generally referred to as a substrate. The substrate is a molecule that will undergo a reaction that results in it splitting into two separate molecules, called products
• The substrate-specific to a particular enzyme will bind at the active site, forming what is known as an enzyme/substrate complex. In other words, a complex formed by the union of the enzyme and substrate
• Hence, the right answer is option E "both A and B"