The answer is letter A. substitution of 3rd N-base to go
from AUU to AUC. Substitution mutations are a type of mutation in which a
single nucleotide is substituted with a different nucleotide. There are many
different ways that DNA can be changed, resulting in different types of
mutation; this are the type of mutations Substitution, Insertion, Deletion, Frameshift.
Answer:
This is not a question we can answer, this is a question only you can answer because it is asking for your personal belief on wether alien's exist outside of earth or not.
Explanation:
Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
<span>As humans change the way we live on the planet, the way that nitrogen moves around the Earth also changes. Nitrogen atoms may seem small enough to be easily overlooked. We look right through the ones in the air, do we not? Yet recent changes in the nitrogen cycle are causing a very noticeable effect on natural environments and human health. Lakes are clogged with aquatic weeds. Dead zones have formed in areas of the oceans where animals can not survive. Air pollutants that contain nitrogen are decreasing air quality and greenhouse gases that contain nitrogen are becoming more common.</span>
Answer:
<h3>С.shear</h3>
Explanation:
A strike-slip fault is a dip-slip fault in which the dip of the fault plane is vertical. Strike-slip faults result from shear stresses .
