Answer:
anaerobic respiration takes place when you demand your muscles to do vigorous work. normally, aerobic respiration takes place but when energy is needed immediately, anaerobic respiration takes place. during anaerobic respiration,the body produces lactic acid, which leads to the burning sensation. your lungs deliver oxygen to the muscles much slower than the rate you need, since you're putting your muscles through an extensive exercise, and this leads to muscle fatigue.
Wave of option A has the highest frequency.
Option A.
<h3><u>Explanation:</u></h3>
Frequency is defined as the total number of complete waves in an unit time. A complete wave consists of a complete cycle of the amplitudes of waves.
In all the figures, we see transverse waves. They have the mean position denoted by a straight line, and the waves do oscillate up and down to complete a full cycle. The most number of waves in a given time has the highest frequency. If we count the full number of waves in the particular given time, Wave A has 5.75 complete waves, wave B has 3.5 complete waves, wave C has 2 complete waves and the wave D has 2.75 complete waves. So the wave A has highest number of complete oscillations in a given time. So wave A has highest frequency.
I think It purifies the soluble GFP in the supernatant by passing over the supernatant over an HIC columnin a highly salty buffer.