When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible restriction enzymes. An enzyme, DNA ligase, then covalently binds the plasmid to the new fragment thereby generating a complete, circular plasmid that can be easily maintained in a variety of biological systems. Read on for an in-depth breakdown of how to do perform restriction digests.
Answer:the resistance that one surface or object encounters when moving over another.
Explanation:it is easy!
What is the question you have?
Answer:
shrinking and I'm trying to keep typing because I need more than 20 characters on my answer
Answer:
Nitrate deficiency
Explanation:
Plants absorb nitrates in water through their roots. Nitrates are present in high levels in plant fertilisers. Without nitrates, the amount of chlorophyll in leaves reduces. This reduces the plant's ability to photosynthesise and grow properly, which reduces the farmers' crop yield .
