Answer:
The correct answer is option A) "Sequence of bases".
Explanation:
Even though DNA most of the time is comprised entirely of only four different nucleotide bases (adenine, thymine, guanosine and citosine), it is the sequence of these bases that allows DNA to carry a vast amount of hereditary information. The genes that carry the hereditary information are translated according to the sequence of the DNA bases. The information is translated by triplets of bases (codons) that correspond to different amino acids that are added to new synthesized proteins during translation.
versatile cas12k-based genetic engineering toolkit (c12kget)
- Genetic modification of micro-organisms is conducive to the selection of high production producers of high-value-added chemicals, but a deficient of genetic tools limits the industrialization of most wild species
- It is very important to develop host-independent gene editing tools which can be used for genetic modification deprived strains.
- A easily found Cas12k-based genetic engineering toolkit (C12KGET) that can result into genomic integration of small parts up to 10 kb in size with up to 100% efficiency in challenging strains.
- By using C12KGET, we accomplished the first example of highly efficient genome editing in Sinorhizobium meliloti, which was successfully solved the question of that industrial strains are difficult to genetically modify, and increased vitamin B12 production by 25%.
- Cas12k can be directly used for transcriptional regulation of genes with up to 92% efficiency due to its naturally inactivated nuclease domain
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CRISPR/Cas9 is the most widely used genome editor.
Genome editing, also called gene editing, is an area of research seeking to modify genes of living organisms to improve our understanding of gene function and develop ways to use it to treat genetic or acquired diseases.
In a wheel and axle, force may be applied either to the wheel or to the axle. This force is called the input force. A wheel and axle does not change the direction of the input force.
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