A is ur answer m8 hope it helps
Answer:
Given:
Number of colonies on plate = 40
Amount of DNA used for transformation = 100ng
Volume of competent cells used = 100ul
Total volume = 1ml (1000ul)
Volume plated for growth = 200ul
The amount of cells transformed per 1 µg of DNA is called the transformation efficiency
(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA
Amount of DNA used for plating = 100ng * (200/1000)
= 100ng * (1/5)
= 100/5 = 20ng
Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng
(2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA
Transformation efficiency = 2000 Transformants / ug DNA
= 2 x 10³ Transformants / ug DNA
Explanation:
Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.
First they minimize the size of their leaf to prevent evaporation, second they have big trunk that could hold a lot of water, and third they have this long root to search water
The answer is c. Inside leaves, there's a lot of chloroplast, which contains chlorophyll for photosynthesis. This can make energy.
Groundwater is defined as water that is found beneath the surface of the earth in conditions of 100 percent saturation (if is less than 100 percent saturation,then the water is considered soil moisture).Ninety-eight percent of earth’s available fresh water is groundwater
