Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
Answer:
Explanation:
mRNA (messanger ribonucleic acid) is produced through the process called transcription in the nucleus. Transcription make ribonucleic acid from deoxyribonucleic acid. The enzyme RNA polymerase creates an RNA molecule that is complementary to the template DNA coping all the information on the DNA into the mRNA formed and 3' to 5' cap are added to stabilize the RNA after which this mRNA then moves from the nucleus where it was produced to the ribosome in the cytoplasm for translation of the information that is coded in the bases.
FGF5 is a gene that is encoded in the DNA.
Answer:
If it helps, the answer is Mutualism (let me know if it is for some reason incorrect)
Answer:
Iron -> Fe - Group 8
Silver -> Ag - Group 11
Mercury -> Hg - Group 12
Oxygen -> O - Group 16
Gold -> Au - Group 11
Potassium -> K - Group 1
Xenon -> Xe - Group 18
Magnesium -> Mg - Group 2
Hydrogen -> H - Group 1
The primary hormone that the thyroid gland releases into the bloodstream is thyroxine. It is the inactive form, and organs like the liver and kidneys convert the majority of it into the active form triiodothyronine.
The body's metabolism, cardiac and digestive processes, muscle control, brain growth, and bone maintenance are all significantly regulated by thyroid hormones.
Hypothyroidism refers to the thyroid gland producing too little thyroxine. It could be brought on by autoimmune conditions, inadequate iodine consumption, or the use of specific medications. Sometimes there is no known cause. Untreated throxine before birth or during infancy can result in mental disability and stunted growth because thyroid hormones are crucial for both physical and mental development.
Adult hypothyroidism results in a slower metabolism. It may cause symptoms like weariness, a diminished ability to tolerate cold conditions, a low heart rate, weight gain, decreased appetite, impaired memory, sadness, muscle stiffness, and decreased fertility. For further details, read the article on hypothyroidism.
To learn more about thyroxine, refer: brainly.com/question/15557539
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