Answer:
Explanation:
Bacterial count in stock- 1.85x10^6 cfu/ml
Dilution methods
Take 100 uL or (0.1ml) from stock and add to 900ul (0.9ml) saline and mixed it- this makes 10^1dilution.
Now take 100ul from 10^1 dilution and add to next 900ul saline this is 10^2 dilution, similarly do upto 10^5 dilution.
Then take 100ul from 10^ 4 and 10^5 dilution seperately and plate on LB agar plate seperetely and count the colonies.
Cfu/ml formula= (No.of colonies x dilution factor)/0.1 ml
So suppose, 18 colonies formed on 10^4 dilution then total no. Of cells in stock will be 18x10^4/ 0.1= 18x10^5 cfu/ml.
If we dilute 10^4 or 10^5 that's leads to colony count of 18-19 colonies on 10^4 dilution while 2 colonies should come on plate of 10^5 dilution.
Chapter 3:
1. Her aunt created a doll out of towels and sowed eyes on the doll.
2. Dr.Alexander Graham Bell was a Scottish born scientist, inventor, engineer, and innovator who was credited with patenting the first practical telephone.
Chapter 4:
1. Because she is blind and deaf.
3. She lacks remorse/sympathy because she is blind and deaf and does not sense the environment.
4. When Miss.Sullivan arrived, Helen was taught to communicate through finger play. She was often frustrated and angry when she could not communicate.
Answer:
In acupressure, pressure points are thought to be powerfully sensitive parts of the body. Some people believe that by applying pressure to our body’s pressure points, it can help relieve pain, establish balance, and improve health throughout the body.
Explanation:
