Answer: Dynamic equilibrium
Explanation:
Answer:
A breakdown of the breaking buffer was first listed with its respective component and their corresponding value; then a table was made for the stock concentrations in which the volume that is being added was determined by using the formula
. It was the addition of these volumes altogether that make up the 0.25 L (i.e 250 mL) with water
Explanation:
Given data includes:
Tris= 10mM
pH = 8.0
NaCl = 150 mM
Imidazole = 300 mM
In order to make 0.25 L solution buffer ; i.e (250 mL); we have the following component.
Stock Concentration Volume to be Final Concentration
added
1 M Tris 2.5 mL 10 mM
5 M NaCl 7.5 mL 150 mM
1 M Imidazole 75 mL 300 mM
. is the formula that is used to determine the corresponding volume that is added for each stock concentration
The stock concentration of Tris ( 1 M ) is as follows:
.

The stock concentration of NaCl (5 M ) is as follows:
.

The stock concentration of Imidazole (1 M ) is as follows:
.

Hence, it is the addition of all the volumes altogether that make up 0.25L (i.e 250 mL) with water.
Answer:
The heat needed to warm 25.3 g of copper from 22°C to 39°C is 165.59 Joules.
Explanation:

Where:
Q = heat absorbed or heat lost
c = specific heat of substance
m = Mass of the substance
ΔT = change in temperature of the substance
We have mass of copper = m = 25.3 g
Specific heat of copper = c = 0.385 J/g°C
ΔT = 39°C - 22°C = 17°C
Heat absorbed by the copper :

The heat needed to warm 25.3 g of copper from 22°C to 39°C is 165.59 Joules.