Transfer of newly created plasmids to e coli. The bacteria that contains a plasmid with antibiotic resistance will grow in the presence of antibiotic concludes that the insertion was successful.
<h3><u>Explanation:</u></h3>
The plasmid DNA that contains the foreign DNA when integrated with the competent bacteria which is e coli and heated, plasmid DNA will be entering into the bacteria by means of some smaller pores that are created in the membranes of the cell.
The plasmid DNA will be copied many times with the help of the replicating mechanism of DNA of the bacteria. Bacteria will grow on a nutrient rich food called agar after the transformation process. Those bacteria that contains a plasmid containing antibiotic resistance only grow in the antibiotic presence. Transformed bacteria will then grow in larger quantity.
