D. Prokaryotes only have one origin of replication to initiate replication.
Explanation:
The DNA of prokaryotes is smaller than that of eukaryotes. So, prokaryotes have only one origin of replication. Origin of replication is the region of DNA where the process of DNA replication starts. The helicases recognize and bind to the origin of replication to start the melting of DNA double helix for replication.
DNA replication starts at the single origin of replication and stops at termination in prokaryotes.
