The bacterial cultivation refers to growing the bacterial species for exploitation or use. But the humans are not able to cultivate the majority of the bacterial species present which could be accounted for many reasons.
The bacteria can mutate very easily through the exchange of genetic material. Some bacterial species cannot be isolated. New bacterial species are discovered in less period of time and some species fail to grow on the growth medium due to lack of information. Till now only one percent of bacterial species have been cultivated.
Genetic engineering is applied to techniques like DNA cloning in which the steps are: step 1: DNA cutting from the parent source; step 2: DNA cut is pasted into the matrix to be modified; step 3: matrix is introduced to the host cell and step 4, vector DNA is isolated. Answer then is B.