Mitophagy refers to the process which is evolutionarily conserved in the organisms to remove the dysfunctional mitochondria in the cell.
The mitochondrial is degraded by the digestive organelles by engulfing the mitochondria.
The mitochondria are engulfed by the lysosome and then phagosome is formed which is double membrane structure.
This phagosome matures into the autophagosome and now has the mitochondria engulfed.
Since the mitochondria contain toe layer and during mitophagy engulfed by the phagosome, therefore, there is a total of four-layer.These four layers separate the matric of the mitochondria with the cytosol.
a possible explanation for this occurrence is that a test tube or dropper was accidentally used repeatedly without being sterilized, causing sample cross-contamination. DNA in this test case did not confirm that the father of the second child was another client of the fertility clinic who had never met the mother...therefore this is a possibility.