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Paul [167]
3 years ago
10

Which type of cell has the potential to develop into any type of cell

Biology
1 answer:
earnstyle [38]3 years ago
7 0

i believe a stem cell is the only type of cell that can grow into any cell. meaning it can go anywhere it may be needed.

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The CRISPR/Cas9 system can cleave genomic DNA at sequences other than the desired target, a phenomenon referred to as off target
Deffense [45]

Answer:

The minimum length of a sgRNA sequence to avoid off target cleavage by the CRISPR/Cas system in the fly fruit genome is 14 bases

Explanation:

We are trying to use the CRISPR/Cas system to cleavage the genome of the fruit fly (which is 1.4x10^8 bp long). Also we desire the cleavage to be unique. That means we need a target sequence long enough to be able to assume it will only appear once in the genome.

First, we should think that in every position, we can find one out of four different nucleotide (A, C, T, G). So, the probability of getting a sequence of a given length "n" will be (1/4)^n (We are assuming that the probability of finding a nucleotide in the position "i", it's independent of the nucleotide we find in any other position "j").

Also, to know how many times a sequence will appear in a genome (the expected value of occurrence), we must multiply the probability of that sequence to randomly occur by the length of the genome. For our specific example, the number of occurence of a sequence of length "n" is:

nºoccurence=[(1/4)^n]*1.4*10^8

But in this case, what we want is the expected number of times the sequence will appear to be 1, and we want to obtain the length of the target sequence (n).

Given the information above, we know that:

[(1/4)^n]*1.4*10^8 =1

[(1/4)^n]=(1/1.4*10^8)=1.4*10^-8

Then, if we want to calculate n, we can use logarithms and its properties to get:

log[(1/4)^n]=log[1.4*10^-8]

n*log[(1/4)]=log[1.4*10^-8]

n=log[1.4*10^-8]/log[(1/4)] => n=13.29 approximately.

As the sequence needs to have a natural number of elements, <u>we can conclude that using a target sequence of a minimum of 14 bases with the CRISPR/Cas system in the fly fruit genome should be enough to avoid off target cleavage.</u>

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What contributes to cell membrane fluidity? Proteins and lipids exist as separate but loosely attached molecules that can move a
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Answer:

Proteins and lipids exist as separate but loosely attached molecules that can move around

Explanation:

Cell membranes are mainly composed of lipids, proteins, and also carbohydrates. Phospholipids are the most abundant type of lipid and the main constituent of the cell membranes. Membrane proteins are divided into two types according to their interactions with the cell membrane: 1-integral (intrinsic) and peripheral (extrinsic) proteins. These peripheral proteins are loosely attached by ionic bonds or calcium bridges with the phosphate heads of the phospholipids; whereas integral membrane proteins contain side chains that interact with fatty acyl groups of the phospholipids. Cell membrane fluidity indicates how easily lipids (e.g., phospholipids and cholesterol) and proteins (e.g., intrinsic proteins) diffuse laterally in the cell membrane. This fluidity is affected by the amount of cholesterol, temperature, and the ratio of unsaturated to saturated fatty acids. Saturated fatty acids have no double bonds in the hydrocarbon chain, whereas unsaturated fatty acids have at least one double bond (these double bonds increase fluidity). Moreover, higher temperatures increase membrane fluidity, whereas cholesterol molecules function to regulate membrane fluidity: at high temperatures cholesterol molecules stabilize the membrane, whereas at low temperatures intercalate between phospholipids, thereby preventing them from clustering together.

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