When something needs to be transported against its concentration gradient, or a molecule is too big to simply diffuse through a membrane, active transport has to be used. So active transport requires energy (ATP).
Examples:
1. Antiport pumps: transport one substance in one direction while transporting another substance the other way. An example of this is the sodium-potassium pump.
2. Symport pumps - uses a substance that wants to move from an area of high concentration to low concentration and carries another substance against its concentration gradient. An example of this is the sodium-glucose transport protein
3. Endocytosis - large amounts of extra cellular fluid can be taken into a cell - needs ATP to bind proteins in the cell membrane and causes them to change their shape...which eventually causes the surrounding of the fluid to be brought in (a vesicle).
4.exocytosis - opposite of above
The DNA is packaged... it starts by being wrapped around special protein molecules (histones). This forms a nucleosome.
It helps the concept of decentralization because its the same anywhere , and it if it happened assist centralization then it would taste the bet in one place and it would taste different/bad everywhete else <span />