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mamaluj [8]
4 years ago
15

Why is it important to stain youngcultures of bacteria with the grain stain?

Biology
1 answer:
uranmaximum [27]4 years ago
8 0

Answer:

Because older cultures of gram-positive bacteria tend to lose their ability to retain crystal-violet in the peptidoglycan of their cell walls and can be confused with gram-negative bacteria.

Explanation:

Gram staining is used to differentiate between two major groups of bacteria. Gram-positive and gram-negative, these bacteria differ in the amount of peptidoglycan in their cell walls. Gram-positive bacteria have a higher amount of peptidoglycan, which absorbs the violet crystal complex used in gram staining, staining them purple/violet. Old cultures of gram-positive bacteria tend to lose the ability to retain the violet crystal and are stained by safranine, staining them red/pink and appear to be gram-negative.

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Explanation:

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Let us assume that the sickle cell allele is represented by S and the normal allele is represented by A.

Now, it is established that the mother has sickle cell anemia, her genotype would be SS. The father does not have sickle cell, his genotype can either be AA or AS.

Assuming the genotype of the father is AA:

          SS   x   AA

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<em>All the children would be normal in this case.</em>

Assuming that the genotype of the father is AS

           SS    x    AS

      AS   SS   AS   SS

<em>50% of the children would have sickle cell while the remaining 50% would be normal.</em>

<u>Therefore, in order to produce children with sickle cell, the father has to be AS, otherwise, he has to be AA.</u>

<u><em /></u>

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