Question

Devise a serial dilution scheme to prepare 1/20, 1/40, and 1/80 dilutions of a disinfectant

Answer 1

Answer:

The serial dilution can be done by half- logarithmic dilution method.

Explanation:

• This is also called the Ten-fold dilution method.
• The process involves the following steps, and the volumes represented are arbitrary.

1. From the concentrated solution of the disinfectant take 1 ml of disinfectant in a falcon tube. 9 ml of water is added to that. Here we get a solution which is  $\frac{1}{10}$ diluted.
2. Take 1 ml of the above $\frac{1}{10}$ diluted solution in another falcon and add 9 ml of water to it. Here we will get a solution which is  $\frac{1}{20}$ diluted.
3. Take 1 ml of the above $\frac{1}{20}$ diluted solution in another falcon and add 9 ml of water to it. Here we will get a solution which is  $\frac{1}{40}$ diluted.
4. Take 1 ml of the above $\frac{1}{40}$ diluted solution in another falcon and add 9 ml of water to it. Here we will get a solution which is  $\frac{1}{80}$ diluted.