The problem with the here is that we might have introduced bias into our sample by failing to randomize the assignment of gender. By pacing the male gender in the treatment group and females into the control group, this might spring up a spurious association in our experiment as a result of a possible confounding variable, gender. Therefore, assignment of subject shouldn't be on the basis of gender.

2.)

Using a coin toss in placing subjects into groups will give a good random assignment, however, since only ten subjects are available and of which 5 will be placed into each group, there is no certainty that there will be equal number of heads and tails during the 10 flips. Alternatively, a random selection of the name of the 10 subjects could be chosen from a raffle.

3.)

Each batch of rat might be homogenous and hence will affect our experiment and definitely our conclusion. It would be best to assign rats from each batch to all treatment groups in other to obtain a good random design

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2 years ago