Answer:
The correct answer is: d. REs activity is best preserved by freezing the RE and thawing it out for brief periods of time as needed.
Explanation:
- Restriction enzymes can be defined as endonucleases that can recognize specific nucleotide sequence in a DNA (Deoxyribonucleic acid) sequence and cause breakage of phosphodiester bond in the DNA at the target site. Hence, it has the capacity of cleaving a DNA sequence.
- These are made up of proteins which are required to be present in the accurately folded conformation in order to function.
- Any form of alteration in the three-dimensional structure of the protein induced due to physical, chemical, mechanical or physiological factors can cause denaturation of the protein which in turn can render the protein non-functional.
- Freezing of restriction enzymes (REs) directly without the use of glycerol will cause the movement of water molecules within the proteins as well layering of water molecules on the protein surface.
- At low temperature these water molecules change to ice crystals which can alter the conformation of the protein or can cause breakage of peptide bonds in the proteins by physical force.
- This can cause the protein to get denatured and hence non-functional.
- Hence, it is never advisable to preserve REs by freezing directly without glycerol.
- Glycerol forms a layer around the protein molecules and prevents the protein molecules from coming into direct contact with the water molecules and hence prevents protein denaturation.
Answer:
The correct answer is the option - mating factor.
Explanation:
Yeast can reproduce sexually by a signaling pathway called the mating factor pathway. Mating in yeast occurs between two haploid yeast cells. In this process, two yeast cells form a diploid cell.
In this process yeast cells secrete a signaling molecule termed as the mating factor that is used to attract them to mating cells to form a diploid cell.
Thus, the correct answer is option - mating factor.
A Safety Data Sheet refers to a detailed information document prepared by a manufacturer of hazardous chemicals. It describes the physical and chemical properties of the product.
<h3>What is
Safety Data Sheet?</h3>
- A Safety Data Sheet refers to a detailed information document prepared by a manufacturer or importer of hazardous chemicals.
- Describes the physical and chemical properties of the product.
- A safety data sheet provides comprehensive information about a chemical, including its properties physical, health, and environmental hazards; and protective measures or safety precautions to be followed in handling, storing, or transporting materials.
- Safety data sheets inform users about product hazards, how to use the product safely, what to expect if recommendations are not followed, how to recognize symptoms of exposure, and what to do in an emergency .
- Safety data sheets provide information about chemicals and help users of those chemicals to make risk assessments.
- They describe hazards arising from chemicals and provide information on handling, storage, and emergency procedures in the event of an accident.
To learn more about Safety Data Sheet from the given link :
brainly.com/question/28244620
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ATP is primarily produced in the Mitochondria.
