This is because there might not be one restriction site bordering the gene to be cloned and the identical restriction site in the plasmid. Since these two restriction enzymes make compatible sticky ends, the insert has a chance of combining with the plasmid. To ensure efficient digestion, the two recognition sites should be more than 10 base pairs apart. If one of the enzymes is a poor cutter or if the sites are separated 10 base pairs or less, the digestions <span>should be performed sequentially</span>
Answer:
extinction is the normal extinction rate while mass extinction is wild spread event that wipes out majority of plants and animals
Execration in man
transport and gutation in plant
They are organisms apart of the biological Kingdom called protista. They are Eukaryotic organisms.
