Question

A DNA fragment with the sticky end sequence TGGCA will bind with another DNA fragment with the sticky end sequence _____

Answer 1

Answer:

A DNA fragment with sticky end sequence TGGCA will bind with another DNA fragment with sticky end sequence ACCGT.

Explanation:

When a DNA strand is separated by the restriction endonuclease, it forms two separate single strands. These strands or cuts are known as sticky ends as they are detached from the complementary pairs.

These cuts of DNA are without complementary pairs and when they find suitable base pair, they get attached to it. These sticky ends are allowed to fix with the complementary base pairs during PCR/ polymerase chain reaction.

They are called sticky ends as they are ready to stick with the complementary base pairs of nucleotides.

Answer 2

Answer: ACCGT

Explanation:

The fragment of a DNA is made of the nitrogenous bases and ribose sugar and a phosphate group.

The base pairs like adenine takes place only with the thymine and guanine with cytosine. This binding is known as the complementary base pair.

The strand TGGCA will bind to the sticky end containing the ACCGT base pair. The whole strand of the DNA has complementary binding.

DNA is the genetic material in all the living beings that carries the genetic material from one generation to another generation.