Question

primers are used to cleave specific regions of the DNA template. In a typical PCR, primers are used to cleave specific regions of the DNA template. True False

Answer 1

Answer:

False

Explanation:

Primers are the small segments of DNA or RNA. Primers do not cleave DNA at specific regions. It is a function of restriction endonucleases. Primers are required during the process of DNA replication as DNA polymerase cannot start the formation of a new DNA strand without preexisting free 3' OH ends. A primer is formed by a primase enzyme. Primers provide the free 3' OH ends to DNA polymerase which in turn extends them to make the new DNA strands. In PCR, primers serve as the same function for the enzyme of DNA strand formation (Taq polymerase).