Question

You are given an experimental problem involving control of a gene's expression in the embryo of a particular species. One of your first questions is whether the gene's expression is controlled at the level of transcription or translation. Which of the followingmight best give you an answer?
A) You explore whether there has been alternative splicing by examining amino acid sequences of very similar proteins.
B) You measure the quantity of the appropriate pre-mRNA in various cell types and find they are all the same.
C) You assess the position and sequence of the promoter and enhancer for this gene.
D) An analysis of amino acid production by the cell shows you that there is an increase.

Answer 1

Answer:

B) You measure the quantity of the appropriate pre-mRNA in various cell types and find they are all the same.

Explanation:

In the above scenario, we are using the control gene and we should know about the control gene is that its expression is either at transcription level or at translation level. For this situation we should examine the pre-mRNA stage of control gene in various cells because it is control gene so it should be present in various cells.

So, the best practice is to quantify the pre-mRNA level of gene then we can effectively solve the experimental problem and find the gene expression either it is at transcription or translation level.