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Alex777 [14]
3 years ago
11

What is the best definition of a conclusion?

Biology
2 answers:
Alex Ar [27]3 years ago
8 0
A possible explanation of a scientific question based on observation
lozanna [386]3 years ago
7 0

an explanation that uses data to support or reject a hypothesis

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An unknown bacterium produces colorless colonies when inoculated onto an EMB plate. Predict what you would see if you inoculated
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The EMB (Eosin Methylene Blue) agar is a selective and differential agar medium. It contains sucrose and lactose as fermentable substrates together with Eosin Y and methylene blue dyes which in combination gives the agar its characteristic color when prepared and serves as a pH indicator as well as an inhibitor of growth of Gram-positive bacteria. Hence, it is primarily used to isolate Gram-negative fecal coliforms while some positive fecal coliforms such as Staphylococci are also able to grow.

These coliforms are of two types:

  • Lactose or sucrose fermenting coliforms form metallic sheens on the agar as a result of acid production and the response of the indicator to the increased acidity.
  • Non-lactose/sucrose fermenters are only able to produce acid by protein deamination. Hence they either form colorless or pinkish colonies on the agar

On the other hand, Mannitol salt agar is a selective and differential agar medium that is primarily used to isolate Staphylococcal bacteria. The presence of sodium chloride in the medium makes it a partial or complete inhibitor of other bacteria.

Hence, an unknown bacterium that forms colorless colonies on EMB will either be a non-lactose fermenting, Gram-negative coliform that will hardly grow on Mannitol salt agar, or a Gram-positive fecal Staphylococcus.

Staphylococci bacteria are of two types:

  • Coagulase-positive will form yellow colonies on the Mannitol salt agar.
  • Coagulase-negative will form red colonies on the Mannitol salt agar.

More about isolating media can be found here: https://brainly.in/question/7238948

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