Question

In an enzyme immunoassay, the enzyme

Answer 1

Answer:

is bound to the constant region of the secondary antibody.

Explanation:

Enzyme immunoassays are the techniques used to detect the presence of antigens with the help of antibodies. Each of the antibody molecules has a constant and variable region.

The primary antibodies are added to the wells. The constant region of the secondary antibody is bound to an enzyme while its variable region is free so that it can bind to the specific antigen.

Addition of substrate to the system is followed by visualization and/or evaluation of antigen as the reaction between enzyme and substrate produce some visible changes such as color change.