Answer:
Electrophoresis is a technique for the separation of molecules according to the mobility of molecules in an electric field.
Explanation:
For the preparation of an agarose gel it is necessary:
1. Agarose powder.
2. TBE buffer (tris, borate and EDTA) in which the agarose dissolves and serves to soak the gel and transmit the electric field.
3. Heat source to mix and melt the agarose in the buffer.
4. Bucket and mold to solidify the gel and immerse it in the buffer.
Steps:
1. On a map, see the amount of TBE buffer to complete the volume of the mold to be filled with the gel.
2. Measure the amount of variable agarose in the concentration at which the gel will be obtained. The more concentration, the higher resolution. For example, a 2% gel would be 2 grams of agarose dissolved in 100 mL of buffer.
3. Mix the agarose with the buffer and fuse the components to obtain the gel. You need a heat source such as a bath at a temperature that allows the agarose to melt or a microwave, which allows you to perform the process more quickly.
4. Cool the gel so that it can be poured into the bucket, the gel is polymerized.
5. Enter the same concentration in the cuvette containing the buffer as the one used to generate the gel.
Saline solution:
NaCl: 9 g
Distilled water sufficient quantity for 1000 mL
Autoclave and store in refrigerator.
1% bleach solution
Sodium Hypochlorite: 10 g
Distilled water, sufficient quantity for 1000 mL
