1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
eduard
3 years ago
11

Design an experiment to answer the question, what is the effect of caffeine on the rate of mitosis in frog eggs? Label: hypothes

is, null hypothesis, independent and dependent variables, control group. Specify material and time amounts and what you will measure.
Biology
1 answer:
s2008m [1.1K]3 years ago
5 0

Answer:

Explanation:

Null Hypothesis: Their will be no effect of the caffeine on the mitosis.

Hypothesis: Caffeine will inhibit the SHSY5Y cell division.

Dependent variable: Various concentration of the caffein compound.

Independent Variable: temperatures of the experimental condition. If their will be any effect of the temperature in mitosis then it will also affect the mitosis of control cells.

Experimental design.

Culture SHSY5Y neuronal cell line in the T-75 culture flask in CO2 incubator at 37 degree celsius. Next day, add cells from T-75 flask in the 24 well plate and allow cells to adhere to the well by incubating plate in CO2 incubator at 37 degree celsius. Add the compound caffeine at different concentration (say 1 microgram, 2 microgram, 3 microgram, 4 microgram) and incubate the plate for 24 hour. Determine the mitotic cells by following assay.

Assay to determine mitotic cell.

The reagent succinimidyl ester of carboxyfluorescein diacetate (CFSE) binds to cytoplasmic proteins. it is fluroscent reagent. And when cell divide due to mitosis, cytoplasm will also divide and hence, the protein which stained with the CFSE reagent will also goes in the daughter cell. Then, the fluorescence of the stained cells can be read on the plate reader.

Prepare the graph of the fluorescence values (Y-axis) verses different concentrations of the caffein (X-axis) and determine the slop of curve to know the rate of mitosis.<u> </u>

<u>Following test and control samples will be used for the assay.</u>

1. SHSY5Y cell line with added caffeine. It is test sample.

2. SHSY5Y cell line added with buffer. This important to know behaviour of cells under experimental conditions.

3. SHSY5Y cell line with solvent used to dissolve compound caffeine. It is important to know the toxicity or any effect of solvent on the mitosis.

You might be interested in
The enzyme amylase can break glycosidic linkages between glucose monomers only if the monomers are the α form. Which of the foll
Phantasy [73]
"Glycogen, starch, and amylopectin" are the ones among the following choices given in the question that amylase could break <span>down. The correct option among all the options that are given in the question is the first option or option "a". I hope that this is the answer that has practically come to your desired help.</span>
7 0
3 years ago
Read 2 more answers
The plasma membrane is how many layers?
stellarik [79]

Answer:

Plasma membrane contains 2 layers

Explanation:

Plasma membrane is extremely pliable structure composed of two layers of phospholids. Cholesterol are also found in between the phospholipids.

6 0
3 years ago
Alsatian gladioli can have purple or yellow flowers which can be either floppy or straight.
den301095 [7]
A is the answer hope this helps
3 0
2 years ago
.
Arlecino [84]
Number 3 is correct
Concept; an abstract idea or general notion
8 0
3 years ago
Read 2 more answers
Which cell structure is mainly responsible for releasing energy from food molecules in some single-celled organisms?
bekas [8.4K]
The energy in food substances is released via chemical reactions that take place within the mitochondria which are present in some single celled organisms.
4 0
3 years ago
Other questions:
  • Which layers are part of the thermosphere check all that apply
    5·2 answers
  • What chemical reaction creates the feeling of lust
    10·2 answers
  • What taxon (classification levels) are represented by the scientific name of an organism? What are the
    7·1 answer
  • During anaphase II in Meiosis, what major event takes place Question 26 options: Sister chromatids are pulled apart. Chromosome
    11·1 answer
  • El sistema muscular humano presenta tres funciones específicas cuales son
    13·1 answer
  • Suicide only has its consequences for the person who attempts suicide
    11·2 answers
  • Explain how it is possible for a compound to have both ionic and covalent bonds
    6·1 answer
  • Look at the blank with the number 15 in the passage. Which of these answers correctly completes the sentence?
    7·1 answer
  • What is the name of this inheritance pattern?
    12·1 answer
  • Once Telophase is complete, the cell has become two identical
    15·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!