1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
eduard
2 years ago
11

Design an experiment to answer the question, what is the effect of caffeine on the rate of mitosis in frog eggs? Label: hypothes

is, null hypothesis, independent and dependent variables, control group. Specify material and time amounts and what you will measure.
Biology
1 answer:
s2008m [1.1K]2 years ago
5 0

Answer:

Explanation:

Null Hypothesis: Their will be no effect of the caffeine on the mitosis.

Hypothesis: Caffeine will inhibit the SHSY5Y cell division.

Dependent variable: Various concentration of the caffein compound.

Independent Variable: temperatures of the experimental condition. If their will be any effect of the temperature in mitosis then it will also affect the mitosis of control cells.

Experimental design.

Culture SHSY5Y neuronal cell line in the T-75 culture flask in CO2 incubator at 37 degree celsius. Next day, add cells from T-75 flask in the 24 well plate and allow cells to adhere to the well by incubating plate in CO2 incubator at 37 degree celsius. Add the compound caffeine at different concentration (say 1 microgram, 2 microgram, 3 microgram, 4 microgram) and incubate the plate for 24 hour. Determine the mitotic cells by following assay.

Assay to determine mitotic cell.

The reagent succinimidyl ester of carboxyfluorescein diacetate (CFSE) binds to cytoplasmic proteins. it is fluroscent reagent. And when cell divide due to mitosis, cytoplasm will also divide and hence, the protein which stained with the CFSE reagent will also goes in the daughter cell. Then, the fluorescence of the stained cells can be read on the plate reader.

Prepare the graph of the fluorescence values (Y-axis) verses different concentrations of the caffein (X-axis) and determine the slop of curve to know the rate of mitosis.<u> </u>

<u>Following test and control samples will be used for the assay.</u>

1. SHSY5Y cell line with added caffeine. It is test sample.

2. SHSY5Y cell line added with buffer. This important to know behaviour of cells under experimental conditions.

3. SHSY5Y cell line with solvent used to dissolve compound caffeine. It is important to know the toxicity or any effect of solvent on the mitosis.

You might be interested in
Which advance in biology resulted from developments in cell technology?
Mariulka [41]

Answer:

Immortal cell line

Explanation:

An immortalised cell line is a population of cells from a multicellular organism which would normally not proliferate indefinitely but, due to mutation, have evaded normal cellular operation and instead can keep undergoing division. The cells can therefore be grown for prolonged periods in vitro

Immortal cell lines are a very important tool for research into the biochemistry and cell biology of multicellular organisms. Immortalised cell lines have also found uses in biotechnology.

4 0
3 years ago
In which scenario could natural selection most obviously occur?
3241004551 [841]
<span>Natural selection is likely when there are stronger selection pressures. For example, a perpetual selection pressure is the fact that organisms have to compete for food and resources, meaning the best adapted ones survive. However, a stronger selection pressure may cause natural selection to occur more obviously. An example of this is when the number of white peppered moths decreased during industrialization in England due to the soot collecting on the trees. The change in population was very fast and obvious.</span>
8 0
3 years ago
Read 2 more answers
A group of habitats makes up a _____. <br> community<br> biome<br> population
alukav5142 [94]
The answer to this question is biome
8 0
2 years ago
Read 2 more answers
I NEED HELP ON KEY CONCEPT!!
levacccp [35]
Spoons are metal and to find metal bonds you need to use I microscope
8 0
2 years ago
Match the chemical reaction with its correct type.
Sonbull [250]

<em><u>hope</u></em><em><u> </u></em><em><u>it</u></em><em><u> </u></em><em><u>will help uh</u></em><em><u>.</u></em><em><u>.</u></em><em><u>.</u></em><em><u>.</u></em><em><u>.</u></em>

6 0
3 years ago
Read 2 more answers
Other questions:
  • Convection in the upper mantle
    9·2 answers
  • Which of the following terms is described as an organism’s tendency to maintain a stable internal environment?
    8·1 answer
  • What best defines potential energy
    5·1 answer
  • A student conducted an experiment to test whether talking to plants would help them to grow faster. The student talked to one gr
    6·1 answer
  • Some bacteria can form on resting structures called
    15·1 answer
  • Which of the following allows a cell to decipher the generic code
    8·1 answer
  • What type of macromolecule provides insulation?
    7·1 answer
  • What are the advantages and disadvantages to shared decision-making?
    6·1 answer
  • Interactions between Na channels and Na -HCO3- cotransporters in the freshwater fish gill MR cell: a model for transepithelial N
    6·1 answer
  • What is the name given to the process by which a parent cell becomes two identical daughter cells?.
    11·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!