The answer would be d.), an increase in the amount of runoff.
Answer: The answer is A, Transcription
Answer:
The correct answer is B. DNA helicase first unwinds the double helix around the +1 site.
Explanation:
The helicase is an enzyme that specifically breaks the hydrogen bonds of the bases in the transcription process. As E. Coli is a prokaryote, then, the transcription only occurs in one point of the circular DNA. The promoter region indicates where the DNA polymerase has to start replicating and the Topoisomerase unwinds the parts of the DNA that are being excessively twisted because of the separation of the two strands.