Question

Which technique is often used to determine the identity and abundance of microorganisms in an environment? (This technique is particularly useful because it does not require culturing the organisms.)

Answer 1

Answer: Fluorescence in situ hybridization (FISH)

Explanation:

Fluorescence in situ hybridization (FISH) is a powerful technique used in the detection of chromosomal abnormalities. Fluorescence in situ Hybridization, is a technique used to detect the presence of specific groups of Bacteria and Archaea microbes.The fluorescent dye allows the cell to be observed under a microscope. The technique allows for the direct quantification of specific types of Bacteria and Archaea in microbial populations without the need to culture the cells in growth media. Only living cells containing sufficient rRNA are detected by FISH. FISH requires no prior knowledge of the environmental conditions of the system..