DNA isolated from water samples collected in six different sites was prepared and analyzed by gel electrophoresis for the presen
ce of carp. The resulting gel is shown. Lanes 1 through 6 contain the DNA from each site. Lanes 7 and 8 represent the negative and positive controls. Based on the banding patterns, how many sites tested positive for carp?
If lane 7 represent the negative control and 8 shows the positive controls (as we may infer by reading that lanes 7 and 8 represent negative and positive controls RESPECTIVELY) then we can say that lanes 1,3,5,6 and 8 tested positive for carp.
Gel electrophoresis can be defined as the process of separation of the macro-molecules and the fragments based on their charge and size. In the gel electrophoresis given, the lane 7 indicates the negative control, and the lane 8 indicates the positive control. The bands formed in the lanes 1,3,5, and 6 corresponds to the ban in the lane 8, which is a positive control. Hence, based on these observations, it can be said that a total of 4 sites tested positive for carp.
The gases and dust particles thrown into the atmosphere during volcanic eruptions have influences on climate. Most of the particles spewed from volcanoes cool the planet by shading incoming solar radiation. The cooling effect can last for months to years depending on the characteristics of the eruption.
