DNA isolated from water samples collected in six different sites was prepared and analyzed by gel electrophoresis for the presen
ce of carp. The resulting gel is shown. Lanes 1 through 6 contain the DNA from each site. Lanes 7 and 8 represent the negative and positive controls.
Based on the banding patterns, how many sites tested positive for carp?
Based on the banding patterns, how many sites tested positive for carp?
2 answers:
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Explanation:
The lipstick from the crime would be crushed and dissolved in an appropriate liquid solvent. The lipstick would then be placed in one of the ink spots on the starting / base line of the paper. Another spot would be created for the lipstick with which it is being compared with.
The paper chromatogram would then be placed vertically on a solvent that acts as the mobile phase. After some time, when the solvent reaches the solvent front of the paper chromatography, by capillary action, the chromatogram can be removed and left to dry.
Bands will have appeared on the chromatography paper formed from the different components of the lipstick. The bands of the lipstick from the crime scene and that with which it is being compared to are observed. If the bands are all aligned, horizontally, then this means it is a complete match. The lipstick must have been the same used at the crime scene.
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