Answer: DNA polymerase I is able to proofread; DNA polymerase III is not.
Explanation:
Escherichia coli is a gram negative anaerobic bacteria that is found in the gastrointestinal tract of and humans and animals. Escherichia coli has 5 types of DNA polymerase.
a. Polymerase I is used to repair any damaged DNA and proof reading
b. Polymerase II is used in proof reading, DNA Replication and repairs.
c. Polymerase III is primarily responsible for DNA replication. It also has the ability to proof read.
d. Polymerase IV is used to change genetic information of Escherichia coli.
e. Polymerase V is used in DNA repair in Escherchia coli.
Answer:
The correct answer would be "The sticky ends of fragmented DNA are joined with vector DNA".
Recombinant DNA or rDNA refers to the DNA that has been artificially formed by joining the fragments of DNA of two or more species.
The fragments are joined by creating the sticky ends in the DNA fragments.
These sticky ends are complementary to each other which helps the fragments to join to each other.
For example, the sticky end of a gene of interest and the sticky end of the vector DNA joined together to form a recombinant vector.
C. Temperature and pH can affect how enzymes work
Enzymes show a maximum activity at an optimum pH and temperature. Changes in the pH or the temperature can cause damage in the structure of the enzymes, if the structure changes the substrate can no longer bind to the enzyme, which is why the activity of the enzymes starts to slow down.
<em>Answer,</em>
<em><u>False</u></em>
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<em><u>Hope This Helps :-)</u></em>
