Law of Segregation is also popularly known as purity of gamete. Law of Segregation is proposed by Mendel’s which stands universal law till date. The law states that allele (alternative form of same gene) pairs separate or segregate at the time of gamete formation.
The gametes form by meiosis or reductional division so, each gamete receives only one allele of trait.
During metaphase I of meiosis the homologous chromososmes gets arranged on the central plate and get attached to the spindle fibres and then anaphase I begins with the separation of each chromosome of the homologue. Each chromosome of a pair moves to opposite poles.