Question

Suppose that an arginine residue in the active site of an enzyme was mutated to alanine. As expected, the alanine mutant was inactive, suggesting that the arginine residue was critical to the catalytic mechanism. Which mutation is most likely to restore wild‑type level of activity to the alanine mutant? A to S

Answer 1

Answer:

Alanine to lysine.

Explanation:

Mutation may be defined as the sudden, heritable change in the genome sequence of an organism. Two main types of mutation are chromosomal mutation and point mutation.

The mutation has occurred in which the arginine is replaced by alanine. The mutation that can restore to wild type is the conversion of alanine with lysine.    Since both arginine and lysine are basic amino acids. They both shows almost similar function and the replacement of lysine with alanine shows the wild type activity.

Thus, the answer is alanine to lysine.