The value of n, the Hill coefficient, for hemoglobin is about 2 to 3 times as great as the value for myoglobin.
Hill Equation
The two closely related equations that help to explain the binding of macromolecules to ligands are called the Hill equation. It helps to quantify the interaction between various ligand binding sites.
Hill coefficient
It is used to describe the cooperativity of ligand binding. It can be positive and negative depending on the value of the Hill coefficient. If the value of the Hill coefficient is more than one then it exhibits positively cooperative binding and if it is less than one then it exhibits negatively cooperative binding. Then there is the noncooperative binding where the Hill coefficient value is one. As for the hemoglobin and myoglobin, the values are,
- Hill coefficient of hemoglobin is 2.7 - 3.
- Hill coefficient of myoglobin is 1.0.
Thus hemoglobin is positively cooperative and myoglobin exhibits noncooperative binding.
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Answer:
The correct answer is <em>A. real-time PCR can measure the amount of DNA amplified as the reaction proceeds, while standard PCR cannot.</em>
Explanation:
PCR (Polymerase Chain Reaction) is a molecular biology technique. Conventional PCR and real-time PCR are diferent types of PCR, and are both used to exponentially amplify DNA molecules. In both types, a polimerase enzyme is employed to sinthesize DNA copies. The main difference is that conventional PCR is qualitative whereas real-time PCR is quantitative. So, real-time PCR permits not only to detect and amplify DNA but also permits to perform a quantification of the target DNA. This is accomplished by measuring a fluorescence signal - which is directly the amount of DNA amplified- during the course of the reaction.
I guess it will be C: atoms and molecules because according to Jhon Dalton's
atomic theory :"all matter is made up of atoms"
and even if we think of other options think it by urself that not all matter is made up of them
The correct option is A.
A basic solution has a high concentration of hydroxide ions more than any type of ion and that is why it is basic in nature. An acidic solution has a higher concentration of hydrogen ions and that is why it is acidic in nature. A neutral solution has equal amount of hydroxide and hydrogen ions. Solutions are usually classified as either basic or acidic based on their hydrogen ion concentration.
