Question

Exposing inner mitochondrial membranes to ultrasonic vibrations will disrupt the membranes. However, the fragments will reseal "inside out." The little vesicles that result can still transfer electrons from NADH to oxygen and synthesize ATP.
These inside-out membrane vesicles will _____.
A) become acidic inside the vesicles when NADH is added
B) become alkaline inside the vesicles when NADH is added
C) make ATP from ADP and Pi if transferred to a pH 4 buffered solution after incubation in a pH 7 buffered solution
D) hydrolyze ATP to pump protons out of the interior of the vesicle to the exterior

Answer 1

Answer:B

Explanation:

This is Because Plasma membranes purified from human red cells were Converted into small vesicles by disruption in alkaline buffer of low ionic strength. Therefore, Most of these vesicles were inside-out.