The CRISPR-Cas9 system is a simple and most versatile genome-editing tool to edit the genome of an organism.
The system consists of two components: "Cas-9 enzyme" which act like molecular scissors to cut the DNA and "Guide RNA or gRNA" which guides the Cas-9 enzyme to the target DNA by binding to the target DNA.
The gRNA consists of 20 bases long RNA sequence which is complementary to the sequence of the target DNA. This binding ensures the right binding and cutting of the target DNA.
