Question

Create an experiment where you would use both a Southern blot and a Northern blot to determine if a newly isolated bacterium contains and actively uses the same catabolic pathway as previously demonstrated. What is the value in doing the Southern blot first? With results from these experiments, how would you conclude if the same pathway is active or not?

Answer 1

Answer:

■Gene sequences would be used to make Probes for both the Southern and Northern blots.

■The probes will be used to view the presence of each gene with the use of isolated genomic DNA obtained from the isolated bacterium

■Each probes hybridized to the genome shows the pathway is isolated and point of the genes were involved in the substrate catabolism

■The carbon source in the isolate is derived from the substrate inducing the catabolic pathway as RNA determine transcripts present

■Probes hybridizing to the same sequences would be used to determine the gene activity for the pathway as seen in the southern one

■since all the genes present in the genome couldn't be identified, the northern would be important to work on

■Catabolic pathway is determined by the same genes. Hence, the need for gene/transcript probes to hybridize to the transcriptome.