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Y_Kistochka [10]
4 years ago
7

A lab technician provides you with a bacterial pellet labeled "G3". When you observe the pellet under UV light, there is little

to no fluorescence. Other than contamination, provide two scientific explanations for what might have happened to cause this outcome.
Biology
1 answer:
umka21 [38]4 years ago
4 0

Answer: The two possible explanation for the outcome are as follows:

Explanation:

1. The bacterial DNA in the pellet was not completely isolated properly, therefore, no fluorescence was observed in the pellet under the exposure of UV radiation.

2. After 3 hours of induction with the IPTG and labeling with the G3. The IPGT might not be able to bound to the bacterial DNA that could show response of fluorescence in the ultraviolet light.

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How many grams are NaCl are required to prepare 200 mL of a 3.10 M solution?
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The mass (in grams) of NaCl required to prepare the solution is 36.27 g

<h3>What is molarity?</h3>

Molarity is defined as the mole of solute per unit litre of solution. Mathematically, it can be expressed as:

Molarity = mole / Volume

<h3>How to determine the mole of NaCl</h3>
  • Volume = 200 mL = 200 / 1000 = 0.2 L
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The mole of NaCl in the solution can be obtained as follow:

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Cross multiply

Mole = Molarity x Volume

Mole of NaCl = 3.1 × 0.2

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<h3>How to determine the mass of NaCl </h3>

We can obtain the mass of NaCl needed to prepare the solution as follow:

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  • Mole of NaCl = 0.62 mole
  • Mass of NaCl = ?

Mole = mass / molar mass

Cross multiply

Mass = mole × molar mass

Mass of NaCl = 0.62 × 58.5

Mass of NaCl = 36.27 g

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