Question

A lab technician provides you with a bacterial pellet labeled "G3". When you observe the pellet under UV light, there is little to no fluorescence. Other than contamination, provide two scientific explanations for what might have happened to cause this outcome.

Answer 1

Answer: The two possible explanation for the outcome are as follows:

Explanation:

1. The bacterial DNA in the pellet was not completely isolated properly, therefore, no fluorescence was observed in the pellet under the exposure of UV radiation.

2. After 3 hours of induction with the IPTG and labeling with the G3. The IPGT might not be able to bound to the bacterial DNA that could show response of fluorescence in the ultraviolet light.